Regulatory
Part:BBa_K817003:Design
Designed by: Anjui Wu Group: iGEM12_NTU-Taida (2012-09-22)
Novel thermal promoter, Phs
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Phs, a thermal promoter, described by Wayne E. Taylor in Cell Press, 1984, can provide quick response to temperature upshift. In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.
Source
ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)